Detached leaf experiment

I ran another repeat of my detached leaf inoculation where I dip inoculate leaves in zoospores suspension and incubate them without wounding for 2 weeks. I spent 8 hours on Labor Day doing this and it looks as though it hasn't worked. This is the fourth time I have set this experiment up and the other 3 times I got good data, although the trends don't exactly match what I expected at least the leaves got infected. This time I was meticulous so I can't figure out why only 9 leaves out of 150 developed lesions. Gah! At least this shows the expected trend but there are so many misses I can't really use this as quantitative data. Perhaps I can just note the observations. So disheartening.