Plant Pathology in the News

It's not a pathogen that I am working on but this is a good example to illustrate the importance of research in plant pathology. Here is a news article from the BBC about a new wave of bacterial infections on horse chestnut trees in the UK caused by Pseudomonas syringae pv. aesculi. The article offers little information about the pathogen or why it has suddenly become a problem. Confusingly the article mentions two other problems facing these trees, an insect pest (moths from Greece) and a fungal pathogen causing leaf blotch, which are separate problems but may be interlinked with the outbreak. For example it is possible the bacteria was introduced by an insect vector or that the moths and fungal infections rendered the tree more susceptible to the bacterial infection either through stress or wounds.

I am currently taking a plant disease diagnosis class in which we have been learning how to systematically diagnose plant problems and their causes. Each week we are given an unknown plant sample and we are to fill in a diagnosis form for it. This involves identifying the host plant, understanding the pattern of the problem on the plant or plant part and in the environment around the plant such as the whole field or wherever the plant was collected from. Then looking at the symptoms and signs of any pathogens or pests or clues to environmental or human issues causing the problems.

One of my samples in this class was a branch of a cherry tree with severe gumming (bleeding cankers). I sucessfully isolated Pseudomonas syringae pv. syringae from a canker and cultured it on Kings B agar. This media provides specific nutrients which Pseudomonas species can utilise to make a pigment which glows under UV light. This is a rather simple and fun diagnostic tool for diseases caused by P. syringae.

This disease on horse chestnuts is of interest as it bears resemblance to epidemics of Dutch Elm Disease, Eastern Filbert (Hazelnut) Blight and Sudden Oak Death which are other diseases which also threaten or have devastated trees populations over wide areas in the past. Management of plant diseases on forest hosts such as these trees is rather different to management of diseases on annual crops for example. The epidemiology of these pathogens varies according to factors of the disease triangle (HOST-PATHOGEN-ENVIRONMENT). For disease to occur there must be a virulent pathogen, a susceptible host and conducive environmental conditions. The spread of this epidemic on horse chestnuts will depend on the mode of pathogen spread, the availability of susceptible host trees and management practices in use.

I could go on about the history of the tree diseases I mentioned and the importance of understanding the pathogens and their mode of spread and the conditions they require but I will save that for another day. I'll be taking a forest insect and disease management class in due course and also a forest pathology class so then I'll be bursting with more fascinating stories to tell you!

the first foliar inoculation

Kenny and Kim helped me with the set up which is far too much for one person to do in a day. We set up a little mobile lab bench at the farm to prepare the inoculation caps.

Nik Grunwald visited us while we were setting up, to see how it was going and he took this photo of me inoculating one plant.

Here is how we worked at the table preparing the caps with inoculum and cotton wool.

Here is the end result, hundreds of plants covered in multicoloured caps and clips. I hope some of them get sick.

Now I'm off on a field trip to Hermiston, OR, camping overnight there as part of my plant disease diagnosis class. This weekend I will be analysing the data from the first dip inoculation experiment in the containment chamber so photos of that will be here shortly.

The big day

Today is the big day for the set up of my first full scale foliar inoculation field experiment. This experiment is designed to run each season of the year for 2 years to allow for epidemiological data to be compared between the 8 different Phytophthora spp being studied, the 2 Rhodie cultivars being used, and the 4 seasons of the year. I will also be running a concurrent root inoculation experiment in the same way which should begin in the next couple of weeks. Pictures coming soon...

Soil inoculum


Here are the jars of inoculum I am preparing to use to inoculate the soil half of the experiment at the farm.

Farm Experiment Site

Here are some photos I took yesterday as the farm site is finally set up for the first experiment to go ahead. There is a shade cloth overhead to keep the plants from getting sun scorch, and also a very nice irrigation system has just been installed. You can see the mist created by the irrigation, which keeps the leaves nice and susceptible to disease! The different cultivars and treatments in the experiment are randomised among each rack of plants.

In this picture you can see the shade cloth overhead and the complicated plumbing for the 6 zones of the structure so that I can program each zone (which are all colour coded!) to come on for a certain time separately!
This is a rain gauge, one of many sensors I have in the plot to record the conditions the plants are experiencing. the rain is funnelled into a small tipping bucket which holds a specific amount, each tip is recorded and the water drips out of the bottom of the bucket.

Fish

Above are my first male and female betta fish or siamese fighting fish, unfortunately the male died soon after I got him and I replaced him with a new one and also replaced the female for fear that she too was sick with the same problem which killed the male. I got an almost Identical pair and tried to mate them but I couldn't get them to do it, the male would make a bubble nest but then they didn't ever put eggs in it. In the end the female died, I don't know what of, I know the water chemistry is fine and I'm feeding them bloodworms which they love. Anyway I am bored with Bettas, they are not that interesting and have to be kept in separate bowls which is annoying. Below is my original male.
This is my newer male who I still have. There is also a trap door snail in here too on the bottom. He slides around the glass keeping it clean!
In a larger bowl I have 2 red capped gold fish and a black moor fish with bulging eyes (see bottom photo):

coming soon

meet my fish - I will soon be posting photos of my new fish and possibly of some plants I am cultivating to turn my room into a mini jungle!

soil inoculation and farm experiments - I will be getting my farm Rhodies next week and I am currently making some infested soil inoculum for root inoculations at the farm in August.

music - I recently found this website which customizes your listening to your music tastes, check it out. pandora radio