I just went to visit the farm and check on things for the first time since the set up as I was rather busy taking down the dip inoculations and I also had to go on a two day field trip to Hermiston.
Here are the first signs of infection since the experiment was set up on Tuesday at the farm! This is great news, because it means that despite the hot conditions and the time it took us to get all the 108 plants inoculated the zoospores survived and infected at least some of the plants!
Checks on the inoculum viability the day after the set up confirmed motile zoospores in at least 5 of the 8 species, and inoculum plated on the selective media PARP grew colonies for 6 of the 8 species. The failures in these two check methods are for different species so overall the 8 species there is at least one confirmation that there were viable infection propagules in the inoculum used the day following the experiment. I.E. for the species which did not grow on PARP there were motile zoospores the following day, and for the ones which were inconclusive when looking for motile zoospores did grow on PARP. This is really encouraging information after all the effort that went into this!
Here are the first signs of infection since the experiment was set up on Tuesday at the farm! This is great news, because it means that despite the hot conditions and the time it took us to get all the 108 plants inoculated the zoospores survived and infected at least some of the plants!
Checks on the inoculum viability the day after the set up confirmed motile zoospores in at least 5 of the 8 species, and inoculum plated on the selective media PARP grew colonies for 6 of the 8 species. The failures in these two check methods are for different species so overall the 8 species there is at least one confirmation that there were viable infection propagules in the inoculum used the day following the experiment. I.E. for the species which did not grow on PARP there were motile zoospores the following day, and for the ones which were inconclusive when looking for motile zoospores did grow on PARP. This is really encouraging information after all the effort that went into this! 
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